SOLVED: Design TWO primers that will be used to amplify the coding sequence of the peptide. Write the primer sequences, melting temperatures, and propose a PCR cycling program/protocol to amplify the gene using LyQuI primers. You should mention temperatures and times for the PCR cycles and steps. Hints: You should include restriction sites in the primers. Your amplified PCR product should be inserted into the plasmid in-frame to allow for successful expression.What enzyme(s) should be used to cu
SOLVED: Design TWO primers that will be used to amplify the coding sequence of the peptide. Write the primer sequences, melting temperatures, and propose a PCR
VIDEO ANSWER: When you amplify a target dena using p c r list of components need to for any standard part given, you're taxed. What do we need now? There is a …
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