Plasmid DNA Extraction (Gigaprep)

Plasmid DNA Extraction (Megaprep)

Plasmid DNA Extraction (Midiprep)

SOLVED:Which of the following facilitates joining a piece of human DNA with bacterial plasmid DNA? a. DNA polymerase c. RNA polymerase b. DNA ligase d. restriction enzymes

You are cloning an aardvark gene, using a bacterial plasmid as a vector. The green diagram shows the plasmid, which contains the restriction site for the enzyme used in Figure 20.6 . Above the plasmid is a segment of linear aardvark DNA that was synthesized using PCR. Diagram your cloning procedure, showing what would happen to these two molecules during each step. Use one color for the aardvark DNA and its bases and another color for those of the plasmid. Label each step and all 5^' and 3^' end

SOLVED:A number of yeast-derived elements were added to the circular bacterial plasmid pBR322. Yeast that require uracil for growth (Ura ^{-} cells) were transformed with these modified plasmids and Ura ^{ } colonies were selected by growth in media lacking uracil. For plasmids containing each of the elements listed in parts (a) to (c), indicate whether you expect the plasmid to integrate into a chromosome by recombination, or instead whether it is maintained separately as a plasmid. If the plas

DNA Cloning The plasmid cloning vector pBR322 (see Fig. 9−3 ) i... | Filo

Expression Cloning | Science Project

Plasmid Cloning by PCR