QIAGEN Plasmid Kits | Plasmid DNA Extraction | QIAGEN
QIAGEN Plasmid Kits provide gravity-flow, anion-exchange tips for purification of transfection-grade plasmid DNA. Lysate clearing and isopropanol precipitation are achieved by centrifugation.
QIAGEN plasmid purification protocols are optimized for use with cultures grown in standard Luria Bertani (LB) medium to a cell density of approximately 3–4 9x 10 cells/mL, which typically corresponds to a pellet wet weight of approximately 3 g/L medium.
QIAGEN Plasmid Mini, Midi, and Maxi Kits (EN)
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QIAGEN plasmid purification protocols are based on a modified alkaline lysis procedure, followed by binding of plasmid DNA to QIAGEN Anion-Exchange Resin under appropriate low-salt and pH conditions.
Qiagen Midi Prep Protocol Procedure 1. Typically you would start by inoculating a 50-100 ml culture with .5-3mls of starter culture and incubating overnight at 37°C for 12–16 h with vigorous shaking (approx. 300 rpm) 2. The next day your culture should be turbid. Centrifuge the bacteria in 50 ml conical tubes
QIAGEN plasmid purification protocols are based on a modified alkaline lysis procedure, followed by binding of plasmid DNA to QIAGEN Anion-Exchange Resin under appropriate low-salt and pH conditions.
This protocol is for preparation of up to 200μg of high- or low-copy plasmid or cosmid DNA using the QIAGEN HiSpeed Plasmid Midi Kit. A final DNA concentration of up to 0.4μg/μL can be expected, if eluting a high-copy plasmid with 500μL of Buffer TE. Add the provided RNase A solution to Buffer P1 before use.
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QIAGEN Midi Plasmid Preps 1. Grow 25-40 ml culture 12-16 hours. Inoculate from a liquid culture that you are sure contains the plasmid you want. Use a 100 ml Erlenmeyer flask that has been previously autoclaved. 2. Transfer the culture to a 50 ml falcon. Spin 10-15 minutes to …
QIAGEN Plasmid Plus Kits | Plasmid DNA Purification | QIAGEN
The design and unique binding chemistry of the QIAGEN Plasmid Plus columns allow a simple bind-wash-elute procedure based on a novel chemistry. This procedure makes these kits the fastest large-scale prep available — 20 minutes for maxi and midi preps.