Isothermal Amplification - NEB
Isothermal amplification methods provide detection of a nucleic acid target sequence in a streamlined, exponential manner, and are not limited by the constraint of thermal cycling. Although these methods can vary considerably, they all share some features in common.
Loop-mediated isothermal amplification - Wikipedia
In contrast to the polymerase chain reaction (PCR) technology, in which the reaction is carried out with a series of alternating temperature steps or cycles, isothermal amplification is carried out at a constant temperature, and does not require a thermal cycler.
The Long and Short of Isothermal Amplification
Isothermal nucleic acid amplification is a technique used to amplify specific DNA or RNA sequences at a constant temperature, typically between 37–65°C, eliminating the need for the thermal cycling equipment utilized in traditional PCR (polymerase chain reaction).
Choosing an Amplification Method: PCR vs Isothermal
2022年1月6日 · In PCR, a thermal cycler changes the reaction temperatures repeatedly to affect the actions of the temperature-dependent reagents; in contrast, an isothermal amplification reaction occurs at a single temperature.
Loop-Mediated Isothermal Amplification - NEB
Loop-mediated isothermal amplification (LAMP) uses 4-6 primers recognizing 6-8 distinct regions of target DNA. A strand-displacing DNA polymerase initiates synthesis and 2 of the primers form loop structures to facilitate subsequent rounds of amplification.
Isothermal Nucleic Acid Amplification - Thermo Fisher Scientific
Isothermal nucleic acid amplification techniques (INAATs) are fast and cycling-free alternatives to PCR, which enable exponential amplification of nucleic acids at constant temperatures. Each INAAT utilizes specific enzymes and reaction conditions, but all require polymerases with strand-displacement activity.
Loop-Mediated Isothermal Amplification (LAMP) | Thermo Fisher ...
Loop-mediated isothermal amplification, also known as LAMP, is a simple and cost-effective nucleic acid amplification technique that does not require expensive instruments and helps ensure rapid results and easy outcome interpretation.
What are the differences between isothermal amplification and PCR ...
2023年5月20日 · Unlike PCR, isothermal amplification does not require a thermal cycler. Instead, it exponentially amplifies target sequences using special strand-displacing DNA and specially designed primer sets. This helps it to overcome limitations inherent in PCR.
What is isothermal amplification? The Polymerase Chain Reaction (PCR) is a well-known approach for amplifying a specific DNA or RNA (RT-PCR) sequence. PCR involves the reiterative cycling of a reaction cocktail between different temperatures to achieve amplification. As routine as PCR is in the molecular biology and molecular diagnostics
Isothermal Amplification - PCR Biosystems
PCR Biosystems’ IsoFast® Bst range of products is based on the large fragment of Bst DNA polymerase and offers rapid and sensitive amplification of DNA and RNA targets at a constant temperature of 65°C. Find the product you need below, or search by application here.