The CRISPR/Cas12a reaction was conducted in a LineGene9600Plus real-time fluorescent quantitative PCR instrument (BIOER, China) maintained at a constant temperature of 37°C, with fluorescence signals ...

This paper reviews the various molecular detection techniques for vegetable soil borne pathogens (PCR, nested-PCR, multiplex PCR, etc.) and their physiological races (host identification, DNA ...

Sec. X), the same length as the DNA used in the random walk model in Fig. 3(c)&(d). In contrast to standard PCR, which uses thermal cycling to separate the strands, we operated the experiment at ...

In this regard, the loop-mediated isothermal amplification (LAMP) method developed by Eiken Chemical Co. Ltd (Japan) has the potential to replace PCR as a result of its simplicity, rapidity ...

PCR-based techniques are methods that rely on the polymerase chain reaction (PCR) to amplify stretches of DNA by creating many identical or near-identical copies. For example, PCR amplification ...

Limited sample material and insufficient DNA input pose significant challenges for downstream analysis in various laboratory settings. To overcome this issue, isothermal amplification techniques have ...

for loop-mediated isothermal amplification. LAMP probably deserves an article of its own to explain the process, but suffice it to say that like PCR, LAMP amplifies nucleic acid sequences ...