The CRISPR/Cas12a reaction was conducted in a LineGene9600Plus real-time fluorescent quantitative PCR instrument (BIOER, China) maintained at a constant temperature of 37°C, with fluorescence signals ...

This paper reviews the various molecular detection techniques for vegetable soil borne pathogens (PCR, nested-PCR, multiplex PCR, etc.) and their physiological races (host identification, DNA ...