In this study, we developed a rapid, sensitive, and visual detection method for MRV and ISKNV using the recombinase polymerase amplification (RPA)−CRISPR/Cas12a system. This method can detect as low ...
Cancer Research Center & Jiangxi Engineering Research Center for Translational Cancer Technology, Jiangxi University of Chinese Medicine & Jiangxi Province Key Laboratory for Diagnosis, Treatment and ...
However, while CRISPR sensing seems a promising alternative to established DNA detection techniques, it is not sensitive to epigenetic changes like hypermethylation. In this presentation, we showcase ...
The team's method uses CRISPR-Cas12a gene editing technology, combined with recombinase polymerase amplification (RPA) and a molecular tool called a "toehold switch" to trigger a visible color ...