The injection site was 2.2 mm lateral to the bregma, 1.0 mm anterior, and 2.7 mm deep. The titer of the ATP5J-KD and ATP5J-OE lentivirus (Gikai Bio, China) was 1E+12 TU/ml. Transfection was achieved ...

For laser doppler measurements, the probe (P403; Perimed, Järfälla, Sweden) was placed 3 mm lateral and 6 mm posterior to the bregma. Relative perfusion units were obtained (Periflux 4001 ...

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For fiber photometry calcium imaging, Tac1-IRES-Cre/+mice were injected with 50-100 nL AAV9-CAG-FLEX-jGCaMP7f into the PSTN (ML ±1.15 mm, AP -2.40 ∼ -2.45 mm, DV - 4.90 mm from bregma). An optic fiber ...

There were nine injection sites: the tip of a glass needle inclined at 30 degrees to the horizontal plane and positioned 5.0 mm laterally (on the injured side) from the bregma, three sites on the ...

(2022). Briefly, 32-channel microwire arrays (Tucker-David Technologies) were surgically implanted to unilaterally target OFC (coordinates: 3.1-4.2 mm anterior, 2.4-4.2 mm lateral relative to bregma; ...