He predicted that the restriction enzyme and the modification enzyme act on the same DNA sequence, called a recognition sequence. In this way, the bacterial cell's own self-defense mechanism ...

The discovery of restriction enzymes in the early 1970s heralded a new age in molecular biology. These enzymes recognize characteristic DNA sequences and cut them. Bacteria and Archaea can also use ...

meaning that the sequence reads the same forward and backward. HindIII, for example, is an H. influenzae restriction enzyme that recognizes the sequence 5'AAGCTT-3' (upper strand)/3'TTCGAA-5 ...

The second key factor for CHiTA helps to overcome this hurdle by removing these added bits of sequence using a protein — called a restriction enzyme — that cuts DNA at specific short sequences ...

This process is referred to as cloning. It uses restriction enzymes to cut the plasmid and the gene fragment at sequence-specific locations that depend on the enzyme used. Then, enzymes called ligases ...