A deeper look into the apparent self-defense mechanisms of these bacteriophage-resistant bacteria revealed their secret weapon: a group of enzymes called restriction endonucleases, or restriction ...

The second key factor for CHiTA helps to overcome this hurdle by removing these added bits of sequence using a protein — called a restriction enzyme — that cuts DNA at specific short sequences ...

Various types of endonucleases – enzymes that can cut DNA – were already known before CRISPR-Cas9. The discovery of restriction enzymes in the early 1970s heralded a new age in molecular biology.

This process is referred to as cloning. It uses restriction enzymes to cut the plasmid and the gene fragment at sequence-specific locations that depend on the enzyme used. Then, enzymes called ligases ...

Lithocholic acid is one such secondary metabolite, made by gut bacteria from a bile acid produced by the host. But the idea ...

The Ti plasmid is removed from the agrobacterium cell, and a restriction enzyme cleaves the T-DNA at a specific restriction site. Subsequently, foreign DNA, which has been cleaved by the same enzyme, ...

A restriction map of the 5.3 kb insert is prepared. The recombinant plasmid is digested with the enzymes listed above in single digests, and then several combinations of enzymes are tested in double ...

Restriction enzymes are one of the most important tools in the recombinant DNA technology toolbox. But how were these enzymes discovered? And what makes them so useful? Aa Aa Aa When I come to the ...