A deeper look into the apparent self-defense mechanisms of these bacteriophage-resistant bacteria revealed their secret weapon: a group of enzymes called restriction endonucleases, or restriction ...

Restriction enzymes are one of the most important tools in the recombinant DNA technology toolbox. But how were these enzymes discovered? And what makes them so useful? Aa Aa Aa When I come to the ...

Various types of endonucleases – enzymes that can cut DNA – were already known before CRISPR-Cas9. The discovery of restriction enzymes in the early 1970s heralded a new age in molecular biology.

A restriction map of the 5.3 kb insert is prepared. The recombinant plasmid is digested with the enzymes listed above in single digests, and then several combinations of enzymes are tested in double ...

The second key factor for CHiTA helps to overcome this hurdle by removing these added bits of sequence using a protein — called a restriction enzyme — that cuts DNA at specific short sequences ...

Researchers find naturally occurring acid induces pseudo-starvation effects, resulting in anti-ageing effects in animals.

Initially, the agrobacterium cell contains a bacterial chromosome and a Tumor-inducing plasmid (Ti plasmid). The Ti plasmid is removed from the agrobacterium cell, and a restriction enzyme cleaves the ...