The PCR cycling reactions are carried out in an automated thermocycler, which is capable of heating and cooling the reaction tubes in a short span of time. PCR-Machines are classified depending on ...
“PCR has a wide range of applications ... cyclers is to remove the variability in temperature control. “Thermal cycling should be the last thing you need to worry about,” says Kok Shyong ...
First, the amount of primer-dimers is variable and depends on numerous factors such as time and temperature before starting PCR cycling, Mg 2+ concentration, the presence of reverse transcriptase ...
Like Molecular Beacons, these primers do not require enzymatic cleavage of the probe during PCR cycling, enabling the option for a post-PCR melt curve analysis. These qualities make Scorpions ...