HindIII, for example, is an H. influenzae restriction enzyme that recognizes the sequence 5'AAGCTT-3' (upper strand)/3'TTCGAA-5' (lower strand) and cleaves between the two A's on both strands.

The eluted DNA will then be used for DNA amplification by the Polymerase Chain Reaction (week 3) 1. Prepare the restriction enzyme digests. You will perform two single digests using enzymes EcoRI and ...