DNA restriction enzymes, with their widespread applications in many diagnostic procedures (e.g., RFLP, PCR, etc), are the very cornerstone of recombinant DNA technology. Unfortunately, their ...

The discovery of restriction enzymes began with a hypothesis. In the 1960s, Werner Arber observed a dramatic change in the bacteriophage DNA after it invaded these resistant strains of bacteria ...

Various types of endonucleases – enzymes that can cut DNA – were already known before CRISPR-Cas9. The discovery of restriction enzymes in the early 1970s heralded a new age in molecular biology.

The second key factor for CHiTA helps to overcome this hurdle by removing these added bits of sequence using a protein — called a restriction enzyme — that cuts DNA at specific short sequences ...

The vectors and copied genes have been treated with restriction enzymes, which are agents that cut DNA sequences at known locations. The enzymes have cut open the round vectors and trimmed the ...

In step 2, the gene fragment is integrated into a circular piece of DNA, called a plasmid, which is the main delivery vehicle into the cell and contains an antibiotic resistance gene for plasmid ...

Initially, the agrobacterium cell contains a bacterial chromosome and a Tumor-inducing plasmid (Ti plasmid). The Ti plasmid is removed from the agrobacterium cell, and a restriction enzyme cleaves the ...